Challenges and opportunities of the China – Gulf Cooperation Council Free Trade Agreement

The free trade agreement between China and the Gulf Cooperation Council (“the GCC”) currently under negotiation is due to become China’s first comprehensive trade and investment agreement with a supranational customs union. The article explores the challenges and opportunities of the proposed China-GCC Free Trade Agreement. It proposes tailor-made recommendations according to the specific interests of both parties

How High Is Too High? Implications of High-Deductible Health Plans

Assesses high deductible health plans, and the likelihood that they would have a substantial effect on either health costs or coverage. Suggests legislative modifications to protect lower wage adults and ensure access to early preventive and primary care

Wages, Health Benefits, and Workers' Health

Examines the divide in the U.S. labor market between higher wage earners with health insurance, and lower wage earners who often lack coverage and access to essential health care

Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.

Alpha1,2-fucosyltransferases, FUT1 and FUT2, which transfer fucoses onto the terminal galactose of N-acetyl-lactosamine via α1,2-linkage have been shown to be highly expressed in various types of cancers. A few studies have shown the involvement of FUT1 substrates in tumor cell proliferation and migration. Lysosome-associated membrane protein 1, LAMP-1, has been reported to carry alpha1,2-fucosylated Lewis Y (LeY) antigens in breast cancer cells, however, the biological functions of LeY on LAMP-1 remain largely unknown. Whether or not its family member, LAMP-2, displays similar modifications and functions as LAMP-1 has not yet been addressed. In this study, we have presented evidence supporting that both LAMP-1 and 2 are substrates for FUT1, but not FUT2. We have also demonstrated the presence of H2 and LeY antigens on LAMP-1 by a targeted nanoLC-MS(3) and the decreased levels of fucosylation on LAMP-2 by MALDI-TOF analysis upon FUT1 knockdown. In addition, we found that the expression of LeY was substantial in less invasive ER+/PR+/HER- breast cancer cells (MCF-7 and T47D) but negligible in highly invasive triple-negative MDA-MB-231 cells, of which LeY levels were correlated with the levels of LeY carried by LAMP-1 and 2. Intriguingly, we also observed a striking change in the subcellular localization of lysosomes upon FUT1 knockdown from peripheral distribution of LAMP-1 and 2 to a preferential perinuclear accumulation. Besides that, knockdown of FUT1 led to an increased rate of autophagic flux along with diminished activity of mammalian target of rapamycin complex 1 (mTORC1) and enhanced autophagosome-lysosome fusion. This may be associated with the predominantly perinuclear distribution of lysosomes mediated by FUT1 knockdown as lysosomal positioning has been reported to regulate mTOR activity and autophagy. Taken together, our results suggest that downregulation of FUT1, which leads to the perinuclear localization of LAMP-1 and 2, is correlated with increased rate of autophagic flux by decreasing mTOR signaling and increasing autolysosome formation

Water research in support of the Sustainable Development Goal 6 : a case study in Belgium

Reaching the Sustainable Development Goal (SDG) 6 on water and sanitation is fundamentally important and conditional to the achievement of all the other SDGs. Nonetheless, achieving this goal by 2030 is challenging, especially in the Global South. Science lies at the root of sustainable development and is a key to new solutions for addressing SDG 6. However, SDG 6-related scientific outputs are often unknown, forming disconnections between academic world and practitioners implementing solutions. This study proposed a bibliometric and text mining method to qualitatively and quantitatively characterize the contribution of water research to the achievement of SDG 6. The method was applied for water research produced by Belgian-affiliated authors with a focus on the Global South collaboration. Despite accounting for less than one percent of the total global publications, Belgian water research has had a relatively high publication rate compared to its neighboring countries. We observed longstanding collaborations between Belgian and scientists from worldwide countries, and an increasing collaboration rate with countries from the Global South. The biggest share of publications focused on topics related to the targets 6.3, 6.4, 6.5, and 6.6, with the main hotspots for Belgian water research being water treatment, water stress, water pollution, climate change, and water modeling. The findings of the bibliometric search have been integrated into an online, user-friendly dashboard to facilitate the identification of research body and experts for practitioners and policy makers. The presented methodology is sufficiently generic and can be used to optimize other science programs in relation to the 2030 Agenda in other countries and regions. In this case study, the findings support shaping the Belgian cooperation and development policy in the water sector, and creating appropriate synergies between Belgian water researchers and their counterparts in the Global South

The need for a definition of big data for nursing science: A case study of disaster preparedness

© 2016 by the author; licensee MDPI, Basel, Switzerland. The rapid development of technology has made enormous volumes of data available and achievable anytime and anywhere around the world. Data scientists call this change a data era and have introduced the term âBig Dataâ, which has drawn the attention of nursing scholars. Nevertheless, the concept of Big Data is quite fuzzy and there is no agreement on its definition among researchers of different disciplines. Without a clear consensus on this issue, nursing scholars who are relatively new to the concept may consider Big Data to be merely a dataset of a bigger size. Having a suitable definition for nurse researchers in their context of research and practice is essential for the advancement of nursing research. In view of the need for a better understanding on what Big Data is, the aim in this paper is to explore and discuss the concept. Furthermore, an example of a Big Data research study on disaster nursing preparedness involving six million patient records is used for discussion. The example demonstrates that a Big Data analysis can be conducted from many more perspectives than would be possible in traditional sampling, and is superior to traditional sampling. Experience gained from the process of using Big Data in this study will shed light on future opportunities for conducting evidence-based nursing research to achieve competence in disaster nursing.Link_to_subscribed_fulltex

Fucosyltransferase 1 and 2 play pivotal roles in breast cancer cells.

FUT1 and FUT2 encode alpha 1, 2-fucosyltransferases which catalyze the addition of alpha 1, 2-linked fucose to glycans. Glycan products of FUT1 and FUT2, such as Globo H and Lewis Y, are highly expressed on malignant tissues, including breast cancer. Herein, we investigated the roles of FUT1 and FUT2 in breast cancer. Silencing of FUT1 or FUT2 by shRNAs inhibited cell proliferation in vitro and tumorigenicity in mice. This was associated with diminished properties of cancer stem cell (CSC), including mammosphere formation and CSC marker both in vitro and in xenografts. Silencing of FUT2, but not FUT1, significantly changed the cuboidal morphology to dense clusters of small and round cells with reduced adhesion to polystyrene and extracellular matrix, including laminin, fibronectin and collagen. Silencing of FUT1 or FUT2 suppressed cell migration in wound healing assay, whereas FUT1 and FUT2 overexpression increased cell migration and invasion in vitro and metastasis of breast cancer in vivo. A decrease in mesenchymal like markers such as fibronectin, vimentin, and twist, along with increased epithelial like marker, E-cadherin, was observed upon FUT1/2 knockdown, while the opposite was noted by overexpression of FUT1 or FUT2. As expected, FUT1 or FUT2 knockdown reduced Globo H, whereas FUT1 or FUT2 overexpression showed contrary effects. Exogenous addition of Globo H-ceramide reversed the suppression of cell migration by FUT1 knockdown but not the inhibition of cell adhesion by FUT2 silencing, suggesting that at least part of the effects of FUT1/2 knockdown were mediated by Globo H. Our results imply that FUT1 and FUT2 play important roles in regulating growth, adhesion, migration and CSC properties of breast cancer, and may serve as therapeutic targets for breast cancer